Publications

2023

George H. Hutchins, Claire E. M. Noble, 
H. Adrian Bunzel, Christopher Williams , Paulina Dubiel, Sathish K. N. Yadav , Paul M. Molinaro, Rob Barringer, Hector Blackburn, Benjamin J. Hardy , Alice E. Parnell, Charles Landau, Paul R. Race , Thomas A. A. Oliver , Ronald L. Koder , Matthew P. Crump , Christiane Schaffitzel , A. Sofia F. Oliveira , Adrian J. Mulholland , and J. L. Ross Anderson. An expandable, modular de novo protein platform for precision redox engineering. PNAS 2023 Jul 24;120 (31) e2306046120.

Hindson SA, Andrews RC, Danson MJ, van der Kamp MW, Manley AE, Sutcliffe OB, Haines TSF, Freeman TP, Scott J, Husbands SM, Blagbrough IS, Anderson JLR, Carbery DR, Pudney CR. Synthetic cannabinoid receptor agonists are monoamine oxidase-A selective inhibitors. FEBS J. 2023 Jun;290(12):3243-3257.

Hardy BJ, Martin Hermosilla A, Chinthapalli DK, Robinson CV, Anderson JLR, Curnow P. Cellular production of a de novo membrane cytochrome. Proc Natl Acad Sci U S A. 2023 Apr 18;120(16):e2300137120.

Day MA, Christofferson AJ, Anderson JLR, Vass SO, Evans A, Searle PF, White SA, Hyde EI. Structure and Dynamics of Three Escherichia coli NfsB Nitro-Reductase Mutants Selected for Enhanced Activity with the Cancer Prodrug CB1954. Int J Mol Sci. 2023 Mar 22;24(6):5987.

2022

Curnow P, Anderson JLR. Expression and In Vivo Loading of De Novo Proteins with Tetrapyrrole Cofactors. Methods Mol Biol. 2022;2397:137-155.

2021

Bunzel HA, Anderson JLR, Hilvert D, Arcus VL, van der Kamp MW, Mulholland AJ (2021) Evolution of dynamical networks enhances catalysis in a designer enzyme. Nat. Chem. 13, 1017-1022. 

Hindson SA, Bunzel HA, Frank B, Svistunenko DA, Williams C, Van Der Kamp MW, Mulholland AJ, Pudney CR, Anderson JLR (2021) Rigidifying a de novo enzyme increases activity and induces a negative activation heat capacity. ACS Catalysis 11, 11532-115431. 

Cheng R, Li J, Rios de Anda I, Taylor TWC, Faers MA, Anderson JLR, Seddon AM, Royall CP (2021) Protein-polymer mixtures in the colloid limit: aggregation, sedimentation and crystallisation. J. Chem. Phys. 155, 114901. 

Bunzel HA, Anderson JLR, Mulholland AJ (2021) Designing better enzymes: insights from directed evolution. Curr. Opin. Struct. Biol. 67, 212-218. 

Jenkins, JMX, Noble CEM, Grayson KJ, Mulholland AJ, Anderson JLR (2021) Substrate promiscuity of a de novo designed peroxidase. J. Inorg. Biochem. 217, 111370. 

Rios De Anda I, Coutable-Pennarun A, Brasnett C, Whitelam S, Seddon A, Russo J, Anderson JLR, Royall CP (2021) Decorated networks of native proteins: nanomaterials with tuneable mesoscopic domain size. Soft Matter17, 6873-6883. 

Day MA, Jarrom D, Christofferson AJ, Graziano AE, Anderson JLR, Searle PF, Hyde EI, White SA (2021) The structures of E. coli NfsA bound to the antibiotic nitrofurantoin; to 1,4-benzoquinone and to FMN. Biochem. J. 478, 2601-2617. 

2020

Le Vay K, Carter BM, Watkins DW, Tang T-YD, Ting VP, Coelfen H, Rambo RP, Smith AJ, Anderson JLR†, Perriman AW (2020) Controlling protein nanocage assembly with hydrostatic pressure. J. Am. Chem. Soc. 142, 20640-20650. 

Curnow P, Hardy BJ, Dufour V, Arthur CJ, Stenner R, Hodgson LR, Verkade P, Williams C, Shoemark DK, Sessions RB, Crump MP, Jones MR, Anderson JLR (2020) Small-residue packing motifs modulate the structure and function of a minimal de novo membrane protein. Scientific Reports 11, 15203. 

Stenner R, Anderson JLR (2020) Chemoselective N-H insertion catalysed by a de novo carbene transferase. Biotech. Appl. Biochem. 67, 527-535.

Ortmayer M, Fisher K, Basran J, Wolde-Michael EM, Heyes DJ, Levy C, Lovelock SL, Anderson JLR, Raven EL, Hay S, Rigby SEJ & Green AP (2020) Rewiring the ‘push-pull’ catalytic machinery of a heme enzyme using an expanded genetic code. ACS Catalysis 10, 2735-2746. 

Stenner R, Steventon JW, Seddon A, Anderson JLR (2020) A de novo peroxidase is also a promiscuous yet stereoselective carbene transferase. Proc. Natl. Acad. Sci. USA 117, 1419-1428. 

2019

Martin N, Tian L, Spencer D, Coutable-Pennarun A, Anderson JLR, Mann S (2019) Photoswitchable phase separation and oligonucleotide trafficking in DNA coacervate microdroplets. Angewandte Chemie Intl. Ed.58, 14594-14598. 

2018

LaLaurie C, Dufour V, Meletiou A, Ratcliffe S, Harland A, Wilson O, Vamasiri C, Shoemark D, Williams C, Arthur C, Sessions RB, Crump MP, Anderson JLR, Curnow P (2018) The de novo design of a biocompatible and functional integral membrane protein using minimal sequence complexity. Scientific Reports 8,14564.

Grayson KG & Anderson JLR (2018) Designed for life: Biocompatible de novo designed proteins and components. J. R. Soc. Interface 15, 20180472. 

Tang T-YD, Cecchi D, Fracasso G, Accardi D, Coutable-Pennarun A, Mansy SS, Perriman AW, Anderson JLR, Mann S (2018) Gene-mediated chemical communication in synthetic protocell communities. ACS Synth. Biol. 7, 339-346. 

Grayson KG & Anderson JLR (2018) The ascent of man(made oxidoreductases). Curr. Opin. Struct. Biol. 51, 140-155. 

2017

Watkins DW, Jenkins JMX, Grayson KJ, Wood N, Steventon JW, Le Vay KK, Goodwin MI, Mullen AS, Bailey HJ, Crump MP, MacMillan F, Mulholland AJ, Cameron G, Sessions RB, Mann S, Anderson JLR (2017) Construction and in vivo assembly of a catalytically proficient and hyperthermostable de novo enzyme. Nat. Commun. 8:358. 

2016

Risbridger TA, Watkins DW, Armstrong JW, Perriman AW, Anderson JLR, Fermin D (2016) Effect of bioconjugation on the reduction potential of heme proteins. Biomacromolecules 17, 3485-3492. 

Armstrong CT, Mason P, Anderson JLR, Dempsey CE (2016) Arginine side chain interactions and the role of arginine as a gating charge carrier in voltage sensitive ion channels. Scientific Reports 6, 21759. 

Watkins DW, Armstrong CT, Beesley JL, Marsh JE, Jenkins JMX, Sessions RB, Mann S, Anderson JLR (2016) A suite of de novo c-type cytochromes for functional oxidoreductase engineering. Biochim. Biophys. Acta Bioenergetics 1857, 493-502. 

2015

Tang T-YD, van Swaay D, deMello A, Anderson JLR, Mann S (2015) In vitro gene expression within membrane-free coacervate protocells. Chem. Commun. 51, 11429-11432.

Armstrong JPK, Shakur R, Horne JP, Dickinson SC, Armstrong CT, Lau K, Kadiwala J, Lowe R, Seddon A, Mann S, Anderson JLR, Perriman AW, Hollander AP (2015) Artificial membrane-binding proteins stimulate oxygenation of stem cells during engineering of large cartilage tissue. Nat. Commun. 6, 7405.

2014

Armstrong CT, Anderson JLR, Denton RM (2014) Studies on the regulation of the human E1 subunit of the 2-oxoglutarate dehydrogenase complex, including the identification of a novel calcium binding site. Biochem. J.459, 369-381. 

Watkins DW, Armstrong CT, Anderson JLR (2014) De novo protein components for oxidoreductase assembly and integration. Curr. Opin. Chem. Biol. 19, 90-928. 

2013

Anderson JLR^‡, Armstrong CT, Kodali G, Lichtenstein BR, Watkins DW, Mancini JA, Boyle AL, Farid TA, Crump MP, Moser CC, Dutton PL (2013) Constructing a man-made oxygen binding c-type cytochrome in vivo: electron transfer, oxygen transport and conversion to a photoactive light harvesting maquette. Chem. Sci. 5, 507-514. 

Farid TA, Kodali G, Solomon LA, Lichtenstein BR, Sheehan MM, Fry BA, Bialas C, Ennist, NM, Siedlecki JA, Zhao Z, Stetz, MA, Valentine KG, Anderson JLR, Discher BM, Wand, AJ, Moser, CC, Dutton PL (2013) Elementary tetrahelical protein design for diverse oxidoreductase functions. Nat. Chem. Biol. 9, 826-833. 

Armstrong CT, Watkins D, Anderson JLR (2013) Constructing manmade enzymes for oxygen activation. Dalton Trans. 42, 3165-3150. 

2012

Lichtenstein BR, Farid TA, Kodali G, Solomon LA, Anderson JLR, Sheehan, MM, Ennist NM, Fry BA, Chobot SE, Bialas C, Mancini JA, Armstrong CT, Zhao Z, Esipova TV, Snell D, Vinogradov SA, Discher BM, Moser CC, Dutton PL (2012) Engineering oxidoreductases: maquette proteins designed from scratch. Biochem. Soc. Trans. 40, 561-566.

2011

Zhang L, Anderson JLR, Ahmed I, Norman JA, Negron C, Mutter AC, Dutton PL, Koder RL (2011) Manipulating heme binding thermodynamics in an artificial oxygen transport protein. Biochemistry 50, 10254-10261.

Li M, Green DC, Anderson, JLR, Binks BP, Mann S (2011) In vitro gene expression and enzyme catalysis in bio-inorganic protocells. Chem. Sci. 2, 1739-1745.

2010

Moser CC, Anderson JLR, Dutton PL, (2010) Guidelines for tunneling in enzymes. Biochim. Biophys. Acta Bioenergetics 1797, 1573-1586.

Davydov RM, Chauhan N, Thackray SJ, Anderson JLR, Papadopoulou ND, Mowat CG, Chapman SK, Raven EL, Hoffman BM, (2010) Probing the ternary complexes of indoleamine and tryptophan 2,3-dioxygenases by cryoreduction EPR and ENDOR spectroscopy. J. Am. Chem. Soc. 132, 5494-5500.

2009

Koder RL, Anderson JLR*, Solomon LA, Reddy KS, Moser CC, Dutton PL (2009) Design and engineering of an O2 transport protein. Nature 458, 305-310.

2008

Anderson JLR, Koder RL, Moser CC, Dutton PL (2008) Controlling complexity and water penetration in functional de novo design. Biochem. Soc. Trans. 36, 1106-1111.

Thackray SJ, Bruckmann C, Anderson JLR, Campbell LP, Xiao R, Zhao L, Mowat CG, Forouhar F, Tong L, Chapman SK (2008) Histidine 55 of tryptophan 2,3-dioxygenase is not an active site base but regulates catalysis by controlling substrate binding. Biochemistry 47, 10677-10684.

Li BR, Anderson JLR, Mowat CG, Miles CS, Reid GA, Chapman SK (2008) Rhodobacter sphaeroides haem protein: a novel cytochrome with nitric oxide dioxygenase activity. Biochem. Soc. Trans. 36, 992-995.

2007

De Laurentis W, Khim L, Anderson JLR, Adam A, Phillips RS, Chapman SK, Van Pee KH, Naismith JH (2007) The second enzyme in Pyrrolnitrin Biosynthetic pathway is related to the heme-dependent dioxygenase superfamily. Biochemistry 46, 12393-12404.

Forouhar F, Anderson JLR*, Mowat CG, Vorobiev SM, Hussain A, Abashidze M, Bruckman C, Thackray SJ, Seetharaman J, Tucker T, Xiao R, Ma LC, Zhao L, Acton TB, Montelione GT, Chapman SK, Tong L (2007) Molecular insights into substrate recognition and catalysis by tryptophan 2,3-dioxygenase. Proc. Natl. Acad. Sci. USA, 104, 473-478.

2006

Anderson JLR, Chapman SK (2006) Molecular mechanisms of enzyme-catalysed halogenation. Mol. Biosyst. 2, 350-357.

2005

Anderson JLR, Chapman SK (2005) Ligand probes for heme proteins. Dalton Trans.7, 13-24. 

2004

Ost TWB, Clark JP, Anderson JLR, Yellowlees LJ, Daff S, Chapman SK (2004) 4-cyanopyridine, a versatile spectroscopic probe for cytochrome P450BM3. J. Biol. Chem. 279, 48876-48882. 

Preprints:

Hutchins GH, Noble CEM, Blackburn H, Hardy B, Landau C, Parnell AE, Yadav S, Williams C, Race PR, Oliveira SF, Crump MP, Berger-Schaffitzel C, Mulholland AJ, Anderson JLR (2020) Precision design of single and multi-heme de novo proteins. bioRxiv

Stenner R, Bunzel HA, Mulholland AJ, Anderson JLR (2020) Beneficial substrate partitioning boosts non-aqueous catalysis in de novo enzyme-alginate beads. bioRxiv

Chapters in edited books:

Curnow P & Anderson JLR (2022) Expression and in vivo loading of de novo proteins with tetrapyrrole cofactors. Methods in Molecular Biology 2397, 137-155. 

Hardy FJ, Ortmayer M, Green AP, Noble CEM, Anderson JLR (2021) Recent advances in understanding, enhancing and creating heme peroxidases. Comprehensive Coordination Chemistry III, Chapter 8.11, 238-253. Eds: Constable EC, Parkin G, Que Jr L. (Elsevier Ltd.) 

Risbridger T, Anderson JLR (2018) Bio-inspired and bioelectrochemical approaches in CO₂ reduction catalysis. Electrochemical Reduction of Carbon Dioxide: Overcoming the Limitations of Photosynthesis, Chapter 2, 17-62. Eds: Marken F & Fermin DJ. (RSC Publishing)